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Cloning and Expression
Enzymes
AlwNI
Supplier Images
AlwNI
AlwNI from New England Biolabs
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Citations:
(5)
Supplier Page
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New England Biolabs for
AlwNI
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
AlwNI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0514S
Concentration
10 u/µL
Quantity
500 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
CAGNNN/CTG
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (5)
(1)
Etoposide promotes DNA loop trapping and barrier formation by topoisomerase II
Nature Chemical Biology
May 1, 2023
Tung T. Le, Meiling Wu, Joyce H. Lee, Neti Bhatt, James T. Inman, James M. Berger, Michelle D. Wang
GTAGCACCAAAGGAAACCATCACCCA with Phusion DNA polymerase (NEB,
M0530S
). The
PCR product
was digested with
AlwNI
(
NEB
,
R0514S
), gel purified and ligated to the 2-kb DNA Y-arms40 using T4 Ligase (NEB,
M0202S
). Single-molecule
(2)
Polarity of the CRISPR roadblock to transcription
Nature Structural & Molecular Biology
December 5, 2022
Porter M. Hall, James T. Inman, Robert M. Fulbright, Tung T. Le, Joshua J. Brewer, Guillaume Lambert, Seth A. Darst, Michelle D. Wang
transcription complex (PTC) mapping, and generated by
PCR
and digested with
AlwNI
(
NEB
, catalog no.
R0514
; Supplementary Table 4). These DNA segments were then each ligated to a pair of dsDNA arms containing
(3)
Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shortening
Nature communications
March 12, 2021
Baxley RM, Leung W, Schmit MM, Matson JP, Yin L, Oram MK, Wang L, Taylor J, Hedberg J, Rogers CB,
et al
.
(
MCM10
exon 3, Hpy199III (NEB R0622);
CDC45
exon 3,
PflMI
(
NEB
R0509
),
XcmI
(
NEB
R0533
) or
AlwNI
(
NEB
R0514
);
MCM4
exon 2,
BglI
(
NEB
R0143
). To generate HCT116 MUS81-/- exon 2 mutant cell lines, each parental
(4)
A Well-Mixed E. coli Genome: Widespread Contacts Revealed by Tracking Mu Transposition
Cell
February 13, 2020
David M. Walker, Peter L. Freddolino, Rasika M. Harshey
(TdT),
New England Biolabs
,
M0315S
HinP1,HinP1,
New England Biolabs
,
R0124S
AlwNI
,
AlwNI
,
New England Biolabs
,
R0514S
BglII
,
BglII
,
New England Biolabs
,
R0144S
T4 DNA ligase,T4 DNA ligase,
New England Biolabs
,
M0202S
Phusion
(5)
Topoisomerase II Is Crucial for Fork Convergence during Vertebrate Replication Termination
Cell Reports
October 8, 2019
Darren R. Heintzman, Lillian V. Campos, Jo Ann W. Byl, Neil Osheroff, James M. Dewar
M280 Streptavidin,
Invitrogen
,
11205D
hTop2a (Topogen),Topogen,TG2000H-1
AlwNI
,
New England Biolabs
,
R0514
XmnI
,
New England Biolabs
,
R0194
Nt.BspQI
,
New England Biolabs
,R0644
Nb.BssSI
,
New England Biolabs
,R0681 Biotin,Sigma,B4501
T4 Polynucleotide Kinase
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AlwNI from New England Biolabs
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